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JoVE Journal Genetics
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
 

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Article DOI: 10.3791/57920-v • 08:22 min • September 15th, 2018
September 15th, 2018 •
1, 2, 3
1Department of Cell and Developmental Biology, University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine, Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Yale School of Medicine

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micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.

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Tissue-specific MiRNA Expression Profiling Mouse Heart Sections In Situ Hybridization Protein Localization MicroRNA Localization Slide Rehydration Hybridization Oven Clearing Agent Proteinase K Working Solution 1x PBS Wash Fixing Tissues 4% PFA Solution Hydrophobic Pen Fixative Removal 0.13 Molar 1-Methylimidazole Wash EDC Fixative
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