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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
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JoVE Journal Genetik
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

DOI:
10.3791/57920-v

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08:22 min

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September 15, 2018

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, ,
1Department of Cell and Developmental Biology,University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine,Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine,Yale School of Medicine

Kapitel

  • 00:04Titel
  • 00:24Tissue Preparation
  • 02:07Probe Hybridization
  • 03:55DIG Antibody Detection
  • 05:20cTNT Antibody Detection and DAPI Staining
  • 06:25Results: PIGF-overexpressing Heart Tissue and miRNA-182 Expression
  • 07:33Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.

Tags

MiRNAIn Situ HybridizationProtein DetectionRehydrationProteinase KFixationHybridizationProbeOptimization

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