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Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
Bölümler
- 00:05Başlık
- 01:02Dissection of Adult Zebrafish Brain
- 03:25Single Cell Dissociation of Adult Brain
- 06:07Generation and Passaging of Primary Neurospheres
- 08:16Results: Analysis of Neurospheres from Adult Zebrafish Brain
- 09:58Conclusion
Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.
