JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
16,065 Views
•
10:42 min
•
October 22, 2012
DOI:
Summary
Automatically generated
NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.
Explore More Videos
Bioluminescence ImagingNADPH Oxidase ActivityAnimal ModelsAntibacterial DefenseAntifungal DefenseInflammatory ResponseReal-time KineticsROS GenerationResearch ToolHost DefenseInflammationInjuryChronic Granulomatous DiseaseCGDInherited DisorderNADPH Oxidase ComponentsGp91phox-deficient MiceP47phox-deficient MiceImpaired Host DefensePhagocytesIsoforms Of NADPH OxidaseQuantify ROS ProductionModels Of Inflammation And Injury
Read Article
Cite this Article
Han, W., Li, H., Segal, B. H., Blackwell, T. S. Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models. J. Vis. Exp. (68), e3925, doi:10.3791/3925 (2012).
Copy