ジャーナル
/
免疫学と感染
/
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

DOI:
10.3791/3925-v

•

10:42 min

•

October 22, 2012

•

, , ,
1医学部,Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology,Roswell Park Cancer Institute, 3医学部,University at Buffalo School of Medicine

章

  • 00:05標題
  • 02:09Animals Models: Intratracheal Administration of Zymosan
  • 04:07Animal Models: Cecal Ligation and Puncture (CLP)
  • 05:15Animal Models: Administration of Oral Carbon Tetrachloride
  • 05:52Acquiring Bioluminescent Images
  • 06:55Data Analysis using Region of Interest
  • 07:45Detection of NADPH Oxidase Mediated ROS Production
  • 09:57Conclusion

概要

自動翻訳

自動翻訳

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

タグ

Bioluminescence ImagingNADPH Oxidase ActivityAnimal ModelsAntibacterial DefenseAntifungal DefenseInflammatory ResponseReal-time KineticsROS GenerationResearch ToolHost DefenseInflammationInjuryChronic Granulomatous DiseaseCGDInherited DisorderNADPH Oxidase ComponentsGp91phox-deficient MiceP47phox-deficient MiceImpaired Host DefensePhagocytesIsoforms Of NADPH OxidaseQuantify ROS ProductionModels Of Inflammation And Injury

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Isolation and Transplantation of Different Aged Murine Thymic Grafts.

Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Read Article