/
/
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
16,072 Views
•
10:42 min
•
October 22, 2012
DOI:
Summary
Automatically generated
NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.
Explore More Videos
Bioluminescence ImagingNADPH Oxidase ActivityAnimal ModelsAntibacterial DefenseAntifungal DefenseInflammatory ResponseReal-time KineticsROS GenerationResearch ToolHost DefenseInflammationInjuryChronic Granulomatous DiseaseCGDInherited DisorderNADPH Oxidase ComponentsGp91phox-deficient MiceP47phox-deficient MiceImpaired Host DefensePhagocytesIsoforms Of NADPH OxidaseQuantify ROS ProductionModels Of Inflammation And Injury
Read Article
Cite this Article
Han, W., Li, H., Segal, B. H., Blackwell, T. S. Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models. J. Vis. Exp. (68), e3925, doi:10.3791/3925 (2012).
Copy