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Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

DOI:
10.3791/3925-v

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10:42 min

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October 22, 2012

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, , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology,Roswell Park Cancer Institute, 3Department of Medicine,University at Buffalo School of Medicine

Kapitel

  • 00:05Titel
  • 02:09Animals Models: Intratracheal Administration of Zymosan
  • 04:07Animal Models: Cecal Ligation and Puncture (CLP)
  • 05:15Animal Models: Administration of Oral Carbon Tetrachloride
  • 05:52Acquiring Bioluminescent Images
  • 06:55Data Analysis using Region of Interest
  • 07:45Detection of NADPH Oxidase Mediated ROS Production
  • 09:57Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

Tags

Bioluminescence ImagingNADPH Oxidase ActivityAnimal ModelsAntibacterial DefenseAntifungal DefenseInflammatory ResponseReal-time KineticsROS GenerationResearch ToolHost DefenseInflammationInjuryChronic Granulomatous DiseaseCGDInherited DisorderNADPH Oxidase ComponentsGp91phox-deficient MiceP47phox-deficient MiceImpaired Host DefensePhagocytesIsoforms Of NADPH OxidaseQuantify ROS ProductionModels Of Inflammation And Injury

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