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Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

16,071 Views

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10:42 min

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October 22, 2012

DOI:

10.3791/3925-v

DOI:
10.3791/3925-v

•

10:42 min
October 22, 2012

•

4 Views
, , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology,Roswell Park Cancer Institute, 3Department of Medicine,University at Buffalo School of Medicine

Summary

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NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

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Cite this Article

Han, W., Li, H., Segal, B. H., Blackwell, T. S. Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models. J. Vis. Exp. (68), e3925, doi:10.3791/3925 (2012).

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