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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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생체공학
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JoVE 신문 생체공학
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

22,125 Views

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08:30 min

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July 18, 2011

DOI:

10.3791/3061-v

DOI:
10.3791/3061-v

•

08:30 min
July 18, 2011

•

9 Views
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1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

Summary

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A tapping mode atomic force microscope (AFM) method for the visualization of plasmid DNA, cytoplasmic proteins, and DNA-protein complexes is described. The method includes alternate approaches for preparing samples for AFM imaging following biochemical manipulation. DNA containing specific protein interacting regions are observed in near-physiologic buffer conditions.

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Murphy, P. J. M., Shannon, M., Goertz, J. Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy. J. Vis. Exp. (53), e3061, doi:10.3791/3061 (2011).

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