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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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July 18, 2011
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A tapping mode atomic force microscope (AFM) method for the visualization of plasmid DNA, cytoplasmic proteins, and DNA-protein complexes is described. The method includes alternate approaches for preparing samples for AFM imaging following biochemical manipulation. DNA containing specific protein interacting regions are observed in near-physiologic buffer conditions.
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Murphy, P. J. M., Shannon, M., Goertz, J. Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy. J. Vis. Exp. (53), e3061, doi:10.3791/3061 (2011).
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