ジャーナル
/
生物工学
/
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
JoVE Journal
生物工学
This content is Free Access.
JoVE Journal 生物工学
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

DOI:
10.3791/3061-v

•

08:30 min

•

July 18, 2011

•

, ,
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

章

  • 00:05標題
  • 01:12Preparing DNA and Protein Samples and Mounting AFM Probe
  • 02:43Preparing the AFM Prior to Sample Imaging
  • 04:33Imaging Mica Sample Surface and Biomolecules of Interest
  • 06:25Representative AFM Images
  • 07:48Conclusion

概要

自動翻訳

自動翻訳

A tapping mode atomic force microscope (AFM) method for the visualization of plasmid DNA, cytoplasmic proteins, and DNA-protein complexes is described. The method includes alternate approaches for preparing samples for AFM imaging following biochemical manipulation. DNA containing specific protein interacting regions are observed in near-physiologic buffer conditions.

タグ

Atomic Force MicroscopyAFMVisualizationRecombinant DNAProtein ComplexesNano-scale ProbeElevation MeasurementMacromoleculesSubstrate SurfaceElectrostatic ForcesTopographical MapThree-dimensional ParticlesTapping Mode AFMBiomaterialsAqueous BuffersImmunoadsorbed ProteinsGlucocorticoid Receptor (GR)Buffered Solution

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Bacterial Immobilization for Imaging by Atomic Force Microscopy

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy

Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Read Article