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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
This content is Free Access.
Chapters
- 00:05Title
- 01:12Preparing DNA and Protein Samples and Mounting AFM Probe
- 02:43Preparing the AFM Prior to Sample Imaging
- 04:33Imaging Mica Sample Surface and Biomolecules of Interest
- 06:25Representative AFM Images
- 07:48Conclusion
A tapping mode atomic force microscope (AFM) method for the visualization of plasmid DNA, cytoplasmic proteins, and DNA-protein complexes is described. The method includes alternate approaches for preparing samples for AFM imaging following biochemical manipulation. DNA containing specific protein interacting regions are observed in near-physiologic buffer conditions.
Tags
Atomic Force MicroscopyAFMVisualizationRecombinant DNAProtein ComplexesNano-scale ProbeElevation MeasurementMacromoleculesSubstrate SurfaceElectrostatic ForcesTopographical MapThree-dimensional ParticlesTapping Mode AFMBiomaterialsAqueous BuffersImmunoadsorbed ProteinsGlucocorticoid Receptor (GR)Buffered Solution
