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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

DOI:
10.3791/3061-v

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08:30 min

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July 18, 2011

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, ,
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

Bölümler

  • 00:05Başlık
  • 01:12Preparing DNA and Protein Samples and Mounting AFM Probe
  • 02:43Preparing the AFM Prior to Sample Imaging
  • 04:33Imaging Mica Sample Surface and Biomolecules of Interest
  • 06:25Representative AFM Images
  • 07:48Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A tapping mode atomic force microscope (AFM) method for the visualization of plasmid DNA, cytoplasmic proteins, and DNA-protein complexes is described. The method includes alternate approaches for preparing samples for AFM imaging following biochemical manipulation. DNA containing specific protein interacting regions are observed in near-physiologic buffer conditions.

Etiketler

Atomic Force MicroscopyAFMVisualizationRecombinant DNAProtein ComplexesNano-scale ProbeElevation MeasurementMacromoleculesSubstrate SurfaceElectrostatic ForcesTopographical MapThree-dimensional ParticlesTapping Mode AFMBiomaterialsAqueous BuffersImmunoadsorbed ProteinsGlucocorticoid Receptor (GR)Buffered Solution

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