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TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis
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Neuroscience
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JoVE Journal Neuroscience
TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

English

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3,225 Views

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09:39 min

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December 16, 2022

DOI:

10.3791/64953-v

DOI:
10.3791/64953-v

•

09:39 min
December 16, 2022

•

11 Views
, , , , ,
1School of Neuroscience,Virginia Polytechnic Institute and State University, 2CMU-Pitt Joint Computational Biology, School of Medicine,University of Pittsburgh, 3Eastern Virginia Medical School, 4Department of Physics,University of Miami

Summary

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TrackMate Analysis of Calcium Imaging (TACI) is an open-source ImageJ plugin for 3D calcium imaging analysis that examines motion on the z-axis and identifies the maximum value of each z-stack to represent a cell's intensity at the corresponding time point. It can separate neurons overlapping in the lateral (x/y) direction but on different z-planes.

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Cite this Article

Omelchenko, A. A., Bai, H., Hussain, S., Tyrrell, J. J., Klein, M., Ni, L. TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis. J. Vis. Exp. (190), e64953, doi:10.3791/64953 (2022).

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