Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

M&#233;gane Bregeon; Nicolas Guyot; Sophie R&#233;hault-Godbert

doi:10.3791/61739

Journal

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Biochemistry

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Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

4,050 Views

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06:12 min

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January 27, 2021

DOI:

10.3791/61739-v

DOI:

10.3791/61739-v

•

06:12 min

January 27, 2021

•

7 Views

Mégane Bregeon¹, Nicolas Guyot¹, Sophie Réhault-Godbert¹

¹Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement,Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

Summary

This article reports a simple method to isolate the outer and inner perivitelline sublayers of chicken eggs while minimizing structural alteration and to optimize protein solubilization of each sublayer for proteomic analyses.

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Perivitelline Membrane Perivitelline Layer Outer Perivitelline Layer Inner Perivitelline Layer Hen’s Eggs Egg Yolk Egg White Chalazae Protein Solubilization Proteomics Mechanical Separation Tris-HCl Buffer Sodium Chloride Freeze-drying Protein Extraction

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Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

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