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Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs
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Bioquímica
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JoVE Revista Bioquímica
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

DOI:
10.3791/61739-v

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06:12 min

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January 27, 2021

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, ,
1Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement,Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

Capítulos

  • 00:05Introduction
  • 00:35PL, IPL, and OPL Samplings
  • 02:44Sample Treatment for Protein Solubilization and SDS-PAGE Analyses
  • 04:37Results: SDS-PAGE Analysis of Separated OPL and IPL from Freshly Laid Unfertilized Egg
  • 05:27Conclusion

Summary

Traducción Automática

Traducción Automática

This article reports a simple method to isolate the outer and inner perivitelline sublayers of chicken eggs while minimizing structural alteration and to optimize protein solubilization of each sublayer for proteomic analyses.

Tags

Perivitelline MembranePerivitelline LayerOuter Perivitelline LayerInner Perivitelline LayerHen’s EggsEgg YolkEgg WhiteChalazaeProtein SolubilizationProteomicsMechanical SeparationTris-HCl BufferSodium ChlorideFreeze-dryingProtein Extraction

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