ジャーナル
/
生化学
/
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs
JoVE Journal
生化学
This content is Free Access.
JoVE Journal 生化学
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

DOI:
10.3791/61739-v

•

06:12 min

•

January 27, 2021

•

, ,
1Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement,Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

章

  • 00:05Introduction
  • 00:35PL, IPL, and OPL Samplings
  • 02:44Sample Treatment for Protein Solubilization and SDS-PAGE Analyses
  • 04:37Results: SDS-PAGE Analysis of Separated OPL and IPL from Freshly Laid Unfertilized Egg
  • 05:27Conclusion

概要

自動翻訳

自動翻訳

This article reports a simple method to isolate the outer and inner perivitelline sublayers of chicken eggs while minimizing structural alteration and to optimize protein solubilization of each sublayer for proteomic analyses.

タグ

Perivitelline MembranePerivitelline LayerOuter Perivitelline LayerInner Perivitelline LayerHen’s EggsEgg YolkEgg WhiteChalazaeProtein SolubilizationProteomicsMechanical SeparationTris-HCl BufferSodium ChlorideFreeze-dryingProtein Extraction

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

Read Article