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Pluripotent Kök Hücrelerden İnsan Kan Damarı Organoidlerinin Üretimi
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
DOI:

09:46 min

January 20, 2023

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Chapters

  • 00:04Introduction
  • 00:52Day 0: Generation of Pluripotent Aggregates from Single-Cell Suspension
  • 02:34Day 1: Mesoderm Induction of the Aggregates
  • 03:41Day 4: Vascular Induction and Priming of the Aggregates
  • 04:20Day 6: Aggregate Embedding and Vessel Sprout Induction
  • 06:02Day 11: Isolation and Maturation of the Blood Vessel Organoids (BVOs)
  • 07:25Results: Stepwise Progression of Human Blood Vessel Organoid Generation from hPSCs
  • 09:13Conclusion

Summary

Automatic Translation

Bu protokol, insan pluripotent ve indüklenmiş pluripotent kök hücrelerden kendi kendini organize eden kan damarlarının oluşumunu tanımlar. Bu kan damarı ağları, perisitler, düz kas aktini ve sürekli bir bazal membran ile çevrili geniş ve bağlı bir endotel ağı sergiler.

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