Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Nicolas Werschler; Josef Penninger

doi:10.3791/64715

Journal

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Bio-ingénierie

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Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Journal JoVE
Bio-ingénierie

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Journal JoVE Bio-ingénierie

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

4,528 Views

•

09:46 min

•

January 20, 2023

DOI:

10.3791/64715-v

DOI:

10.3791/64715-v

•

09:46 min

January 20, 2023

•

54 Views

Nicolas Werschler^1,2, Josef Penninger^1,2,3,4

¹School of Biomedical Engineering,University of British Columbia, ²Life Sciences Institute,University of British Columbia, ³Department of Medical Genetics,University of British Columbia, ⁴Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

Summary

This protocol describes the generation of self-organizing blood vessels from human pluripotent and induced pluripotent stem cells. These blood vessel networks exhibit an extensive and connected endothelial network surrounded by pericytes, smooth muscle actin, and a continuous basement membrane.

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Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

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