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Génération d’organoïdes de vaisseaux sanguins humains à partir de cellules souches pluripotentes
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
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Génération d’organoïdes de vaisseaux sanguins humains à partir de cellules souches pluripotentes

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4,532 Views

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09:46 min

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January 20, 2023

DOI:

10.3791/64715-v

DOI:
10.3791/64715-v

•

09:46 min
January 20, 2023

•

54 Views
,
1School of Biomedical Engineering,University of British Columbia, 2Life Sciences Institute,University of British Columbia, 3Department of Medical Genetics,University of British Columbia, 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

Summary

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Ce protocole décrit la génération de vaisseaux sanguins auto-organisés à partir de cellules souches pluripotentes et pluripotentes induites humaines. Ces réseaux de vaisseaux sanguins présentent un réseau endothélial étendu et connecté entouré de péricytes, d’actine musculaire lisse et d’une membrane basale continue.

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Cite this Article

Werschler, N., Penninger, J. Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells. J. Vis. Exp. (191), e64715, doi:10.3791/64715 (2023).

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