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Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

4,528 Views

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09:46 min

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January 20, 2023

DOI:

10.3791/64715-v

DOI:
10.3791/64715-v

•

09:46 min
January 20, 2023

•

54 Views
,
1School of Biomedical Engineering,University of British Columbia, 2Life Sciences Institute,University of British Columbia, 3Department of Medical Genetics,University of British Columbia, 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

Summary

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This protocol describes the generation of self-organizing blood vessels from human pluripotent and induced pluripotent stem cells. These blood vessel networks exhibit an extensive and connected endothelial network surrounded by pericytes, smooth muscle actin, and a continuous basement membrane.

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Cite this Article

Werschler, N., Penninger, J. Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells. J. Vis. Exp. (191), e64715, doi:10.3791/64715 (2023).

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