Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Nicolas Werschler; Josef Penninger

doi:10.3791/64715

Journal

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Bioengineering

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Generación de organoides de vasos sanguíneos humanos a partir de células madre pluripotentes

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

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Generación de organoides de vasos sanguíneos humanos a partir de células madre pluripotentes

4,532 Views

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09:46 min

•

January 20, 2023

DOI:

10.3791/64715-v

DOI:

10.3791/64715-v

•

09:46 min

January 20, 2023

•

54 Views

Nicolas Werschler^1,2, Josef Penninger^1,2,3,4

¹School of Biomedical Engineering,University of British Columbia, ²Life Sciences Institute,University of British Columbia, ³Department of Medical Genetics,University of British Columbia, ⁴Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

Summary

Este protocolo describe la generación de vasos sanguíneos autoorganizados a partir de células madre pluripotentes humanas y pluripotentes inducidas. Estas redes de vasos sanguíneos exhiben una red endotelial extensa y conectada rodeada de pericitos, actina del músculo liso y una membrana basal continua.

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Blood Vessel Organoids Pluripotent Stem Cells Vasculogenesis Angiogenesis Vascular Disease Vascular Pathologies Reproducibility High Throughput Stem Cell Lines Diabetic Vasculopathy Aggregates Cell Dissociation Aggregation Medium Single-cell Suspension Cell Viability Mesoderm Media

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Generación de organoides de vasos sanguíneos humanos a partir de células madre pluripotentes

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