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Trasplante cardíaco cervical heterotópico de ratón utilizando manguitos vasculares
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Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs
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Trasplante cardíaco cervical heterotópico de ratón utilizando manguitos vasculares

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2,599 Views

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08:44 min

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June 23, 2022

DOI:

10.3791/64089-v

DOI:
10.3791/64089-v

•

08:44 min
June 23, 2022

•

9 Views
, , , , ,
1Department of Surgery,Washington University School of Medicine, 2Department of Surgery,The University of Maryland, 3Department of Pathology & Immunology,Washington University School of Medicine, 4Department of Medicine,Washington University School of Medicine

Summary

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Los modelos de trasplante cardíaco de ratón representan valiosas herramientas de investigación para estudiar la inmunología del trasplante. El presente protocolo detalla el trasplante cardíaco cervical heterotópico de ratón que implica la colocación de manguitos en la arteria carótida común del receptor y el tronco de la arteria pulmonar del donante para permitir el flujo sanguíneo laminar.

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Cite this Article

Li, W., Shepherd, H. M., Krupnick, A. S., Gelman, A. E., Lavine, K. J., Kreisel, D. Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs. J. Vis. Exp. (184), e64089, doi:10.3791/64089 (2022).

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