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Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

DOI:
10.3791/64089-v

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08:44 min

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June 23, 2022

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, , , , ,
1Department of Surgery,Washington University School of Medicine, 2Department of Surgery,The University of Maryland, 3Department of Pathology & Immunology,Washington University School of Medicine, 4Department of Medicine,Washington University School of Medicine

Kapitel

  • 00:04Introduction
  • 01:01Donor Procedure
  • 03:39Recipient Procedure
  • 07:15Results: Using Vascular Cuffs for Heterotopic Cervical Cardiac Transplantation in Mice
  • 08:12Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Mouse cardiac transplantation models represent valuable research tools for studying transplantation immunology. The present protocol details mouse heterotopic cervical cardiac transplantation that involves the placement of cuffs on the recipient's common carotid artery and the donor's pulmonary artery trunk to allow for laminar blood flow.

Tags

Mouse Heterotopic Cervical Cardiac TransplantationVascular CuffsLaminar Blood FlowThrombosis PreventionIschemia-reperfusion InjuryGraft RejectionMicrosurgical TechniqueRetrograde PerfusionAortic Arch DissectionPulmonary Artery Cuff

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