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Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs
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Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

2,599 Views

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08:44 min

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June 23, 2022

DOI:

10.3791/64089-v

DOI:
10.3791/64089-v

•

08:44 min
June 23, 2022

•

9 Views
, , , , ,
1Department of Surgery,Washington University School of Medicine, 2Department of Surgery,The University of Maryland, 3Department of Pathology & Immunology,Washington University School of Medicine, 4Department of Medicine,Washington University School of Medicine

Summary

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Mouse cardiac transplantation models represent valuable research tools for studying transplantation immunology. The present protocol details mouse heterotopic cervical cardiac transplantation that involves the placement of cuffs on the recipient's common carotid artery and the donor's pulmonary artery trunk to allow for laminar blood flow.

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Mouse Heterotopic Cervical Cardiac TransplantationVascular CuffsLaminar Blood FlowThrombosis PreventionIschemia-reperfusion InjuryGraft RejectionMicrosurgical TechniqueRetrograde PerfusionAortic Arch DissectionPulmonary Artery Cuff

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Cite this Article

Li, W., Shepherd, H. M., Krupnick, A. S., Gelman, A. E., Lavine, K. J., Kreisel, D. Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs. J. Vis. Exp. (184), e64089, doi:10.3791/64089 (2022).

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