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Intravital widefield fluorescensmikroskopi av lungemikrosirkulasjon i eksperimentell akutt lungeskade ved hjelp av et vakuumstabilisert bildebehandlingssystem
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1Department of Physiology and Biophysics,Dalhousie University, 2Department of Microbiology and Immunology,Dalhousie University, 3Department of Neuroscience,Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine,Dalhousie University, 5Live Cell Imaging Center,University of Calgary, 6Department of Physiology and Pharmacology,University of Calgary, 7Department of Biochemistry and Molecular Biology,University of Calgary, 8Department of Pharmacology,Dalhousie University
Chapters
- 00:04Introduction
- 00:36Preparation of the Vacuum-Stabilized Lung Imaging System
- 01:10Intubation
- 01:57Ventilation
- 02:47Thoracotomy
- 05:10Microscopy
- 06:56Results: Leukocyte Trafficking and Capillary Perfusion in the Pulmonary Microcirculation
- 08:48Conclusion
Intravital fluorescensmikroskopi kan brukes til å studere leukocytt-endotelinteraksjoner og kapillær perfusjon i sanntid. Denne protokollen beskriver metoder for å avbilde og kvantifisere disse parametrene i lungemikrosirkulasjonen ved hjelp av et vakuumstabilisert lungeavbildningssystem.
