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Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

English

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13,820 Views

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08:30 min

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March 15, 2018

DOI:

10.3791/56685-v

DOI:
10.3791/56685-v

•

08:30 min
March 15, 2018

•

9 Views
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1Translational Experimental Pediatrics, Experimental Pulmonology, Department of Pediatrics and Adolescent Medicine,University of Cologne, 2Department of Pediatrics and Adolescent Medicine,University of Cologne, 3Division of Experimental Pediatrics and Metabolism, University Children’s Hospital,Heinrich Heine University Düsseldorf

Summary

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The incidence of obesity is rising and increases the risk of chronic lung diseases. To establish the underlying mechanisms and preventive strategies, well-defined animal models are needed. Here, we provide three methods (glucose-tolerance-test, body plethysmography, and lung fixation) to study the effect of obesity on pulmonary outcomes in mice.

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Intraperitoneal Glucose Tolerance TestLung Function MeasurementLung FixationObesityImpaired MetabolismPulmonary OutcomesRespiratory ResearchMetabolic DisordersLung StructureLung FunctionChronic Lung DiseasesTherapeutic ApproachesCOPDAsthmaFasted Blood GlucoseGlucose InjectionLung Function AssessmentAnesthesiaAnimal Models

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Dinger, K., Mohr, J., Vohlen, C., Hirani, D., Hucklenbruch-Rother, E., Ensenauer, R., Dötsch, J., Alejandre Alcazar, M. A. Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes. J. Vis. Exp. (133), e56685, doi:10.3791/56685 (2018).

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