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09:28 min
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April 06, 2022
DOI:
1Department of Physiology and Biophysics,Dalhousie University, 2Department of Microbiology and Immunology,Dalhousie University, 3Department of Neuroscience,Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine,Dalhousie University, 5Live Cell Imaging Center,University of Calgary, 6Department of Physiology and Pharmacology,University of Calgary, 7Department of Biochemistry and Molecular Biology,University of Calgary, 8Department of Pharmacology,Dalhousie University
Summary
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생체 내 형광 현미경 검사는 백혈구 - 내피 상호 작용 및 모세관 관류를 실시간으로 연구하는 데 활용 될 수 있습니다. 이 프로토콜은 진공-안정화된 폐 영상화 시스템을 사용하여 폐 미세순환에서 이러한 파라미터를 영상화하고 정량화하는 방법을 기술한다.
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Hall, S., Faridi, S., Euodia, I., Tanner, S., Chojnacki, A. K., Patel, K. D., Zhou, J., Lehmann, C. Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System. J. Vis. Exp. (182), e63733, doi:10.3791/63733 (2022).
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