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使用真空稳定成像系统的实验性急性肺损伤中肺微循环的活体内宽视场荧光显微镜
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System
DOI:

09:28 min

April 06, 2022

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Chapters

  • 00:04Introduction
  • 00:36Preparation of the Vacuum-Stabilized Lung Imaging System
  • 01:10Intubation
  • 01:57Ventilation
  • 02:47Thoracotomy
  • 05:10Microscopy
  • 06:56Results: Leukocyte Trafficking and Capillary Perfusion in the Pulmonary Microcirculation
  • 08:48Conclusion

Summary

Automatic Translation

活体荧光显微镜可用于实时研究白细胞 - 内皮相互作用和毛细血管灌注。该协议描述了使用真空稳定的肺成像系统在肺微循环中对这些参数进行成像和量化的方法。

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