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Vakumla Stabilize Edilmiş Bir Görüntüleme Sistemi Kullanılarak Deneysel Akut Akciğer Hasarında Pulmoner Mikrosirkülasyonun İntravital Geniş Alan Floresan Mikroskopisi
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1Department of Physiology and Biophysics,Dalhousie University, 2Department of Microbiology and Immunology,Dalhousie University, 3Department of Neuroscience,Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine,Dalhousie University, 5Live Cell Imaging Center,University of Calgary, 6Department of Physiology and Pharmacology,University of Calgary, 7Department of Biochemistry and Molecular Biology,University of Calgary, 8Department of Pharmacology,Dalhousie University
Chapters
- 00:04Introduction
- 00:36Preparation of the Vacuum-Stabilized Lung Imaging System
- 01:10Intubation
- 01:57Ventilation
- 02:47Thoracotomy
- 05:10Microscopy
- 06:56Results: Leukocyte Trafficking and Capillary Perfusion in the Pulmonary Microcirculation
- 08:48Conclusion
İntravital floresan mikroskopi, lökosit-endotel etkileşimlerini ve kılcal perfüzyonu gerçek zamanlı olarak incelemek için kullanılabilir. Bu protokol, vakumla stabilize edilmiş bir akciğer görüntüleme sistemi kullanarak pulmoner mikrosirkülasyondaki bu parametreleri görüntüleme ve ölçme yöntemlerini açıklar.
