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检测白伊蚊细胞系中的沃尔巴克氏体菌株 wAlbB
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines
DOI:

08:08 min

June 01, 2022

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Chapters

  • 00:04Introduction
  • 00:43Cell Culture and DNA Extraction
  • 01:55Detection of Wolbachia Nucleic Acid and Quantitative PCR Analysis
  • 02:53Protein Extraction
  • 04:26Immunofluorescence Localization of Wolbachia Protein
  • 06:05Results: Detection of Intracellular Wolbachia Infection at the Nucleic acid and Protein Levels
  • 07:21Conclusion

Summary

Automatic Translation

采用4种方法检测细胞内沃尔巴克氏体,相辅相成,提高了抗生素治愈白纹伊蚊来源的Aa23和Aa23-T感染的细菌感染的准确性。

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