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Detecting Cortex Fragments During Bacterial Spore Germination
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detecting Cortex Fragments During Bacterial Spore Germination

Detecting Cortex Fragments During Bacterial Spore Germination

DOI:
10.3791/54146-v

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08:35 min

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June 25, 2016

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1Department of Biology,Texas A&M University

Chapters

  • 00:05Title
  • 00:34Generating Samples
  • 03:20Measuring Cortex Fragments
  • 05:02Addition of Color Reagent
  • 06:46Results: Representative Germination Assay
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

Herein, we describe a colorimetric assay to detect the presence of reducing sugars during bacterial spore germination.

Tags

Bacterial Spore GerminationCortex FragmentsReducing SugarsClostridium DifficileGermination SolutionOD600CentrifugationN-acetylglucosamineDipicolinic Acid

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