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ヒトスジシマカ細胞株における ボルバキアwAlbBの 検出
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines
DOI:

08:08 min

June 01, 2022

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Chapters

  • 00:04Introduction
  • 00:43Cell Culture and DNA Extraction
  • 01:55Detection of Wolbachia Nucleic Acid and Quantitative PCR Analysis
  • 02:53Protein Extraction
  • 04:26Immunofluorescence Localization of Wolbachia Protein
  • 06:05Results: Detection of Intracellular Wolbachia Infection at the Nucleic acid and Protein Levels
  • 07:21Conclusion

Summary

Automatic Translation

細胞内ボルバキアの検出には4つの方法を用い、抗生物質を用いた天然型ボルバキア感染症を治したヒトスジシマカ由来Aa23とAa23-Tのボルバキア感染の検出精度を向上させ、互いに補完し合いました。

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