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Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

English

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39,416 Views

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06:38 min

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November 08, 2011

DOI:

10.3791/3321-v

DOI:
10.3791/3321-v

•

06:38 min
November 08, 2011

•

17 Views
, , , ,
1Stony Brook Children’s Hospital,State University of New York at Stony Brook, 2Department of Pediatrics,State University of New York at Stony Brook, 3Department of Molecular Genetics,State University of New York at Stony Brook, 4Department of Microbiology,State University of New York at Stony Brook

Summary

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We describe a method for generating transformed B cell lines using Epstein-Barr virus. We also illustrate a novel assay that can identify B cells destined to undergo transformation as early as three days after infection.

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Epstein-Barr VirusGrowth-transformed Lymphoblastoid Cell LinesEBV InfectionB Cell ProliferationImmortalizationEBV LatencyVirus-driven B Cell ProliferationTumorigenesisImmunologic AssaysHuman Monoclonal AntibodiesMitogensPhytohemagglutininLipopolysaccharidePokeweed MitogenCyclosporin AT Cell-mediated KillingHigh Titer EBVImmunosuppressive AgentPeripheral Blood Mononuclear Cells

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Hui-Yuen, J., McAllister, S., Koganti, S., Hill, E., Bhaduri-McIntosh, S. Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines. J. Vis. Exp. (57), e3321, doi:10.3791/3321 (2011).

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