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Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes
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August 13, 2020
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A protocol to culture the microsporidian parasite Edhazardia aedis. The parasite is passaged from one generation of Aedes aegypti mosquitoes to the next via horizontal transfer at the larval stage followed by vertical transmission at the adult stage. Live sporoplasms survive long-term in infected eggs.
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Grigsby, A., Kelly, B. J., Sanscrainte, N. D., Becnel, J. J., Short, S. M. Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes. J. Vis. Exp. (162), e61574, doi:10.3791/61574 (2020).
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