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Immunology and Infection
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Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes

Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes

DOI:
10.3791/61574-v

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05:29 min

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August 13, 2020

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, , , ,
1Department of Entomology,The Ohio State University, 2USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology

Chapters

  • 00:04Conclusion
  • 00:39Days 0 1: Hatch Ae. aegypti Eggs Infected with E. aedis
  • 01:23Days 4-5: Hatch Uninfected Ae. aegypti
  • 01:49Days 7 8: Horizontal Transmission
  • 03:45Results: Effective E. aedis Infection in Filial Generation
  • 05:00Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to culture the microsporidian parasite Edhazardia aedis. The parasite is passaged from one generation of Aedes aegypti mosquitoes to the next via horizontal transfer at the larval stage followed by vertical transmission at the adult stage. Live sporoplasms survive long-term in infected eggs.

Tags

MicrosporidianEdhazardia AedisAedes AegyptiParasite PropagationMosquito RearingSpore QuantificationLarval InfectionSpore InoculationLarval Food Slurry

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