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Immunology and Infection
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Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines

Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines

DOI:
10.3791/63662-v

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08:08 min

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June 01, 2022

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, , , ,
1Guizhou Medical University, 2Guizhou Health Vocational College

Chapters

  • 00:04Introduction
  • 00:43Cell Culture and DNA Extraction
  • 01:55Detection of Wolbachia Nucleic Acid and Quantitative PCR Analysis
  • 02:53Protein Extraction
  • 04:26Immunofluorescence Localization of Wolbachia Protein
  • 06:05Results: Detection of Intracellular Wolbachia Infection at the Nucleic acid and Protein Levels
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

Four methods were used to detect intracellular Wolbachia, which complemented each other and improved the detection accuracy of Wolbachia infection of Aedes albopictus-derived Aa23 and Aa23-T cured of native Wolbachia infection using antibiotics.

Tags

WolbachiaAedes AlbopictusCell LinesPCRQPCRWestern BlotProtein AssayDNA ExtractionCell Culture

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