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完整小鼠和斑马鱼大脑的深组织三光子荧光显微镜
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
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完整小鼠和斑马鱼大脑的深组织三光子荧光显微镜

DOI:
10.3791/63213-v

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08:26 min

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January 13, 2022

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, , , , , , , , ,
1School of Applied and Engineering Physics,Cornell University, 2Department of Neurobiology and Behavior,Cornell University, 3College of Veterinary Medicine,Cornell University, 4Meinig School of Biomedical Engineering,Cornell University

Chapters

  • 00:05Introduction
  • 01:04Three-Photon Microscopy Setup
  • 02:01Fish Preparation for Three-Photon Imaging
  • 03:52Intravital Imaging in Fish
  • 05:50Intravital Imaging in Mouse
  • 06:39Results: Three-Photon Imaging in Intact Mouse and Zebrafish Brain with High Spatiotemporal Resolution
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

三光子显微镜能够以高时空分辨率在活生物组织（如小鼠和斑马鱼大脑）深处进行高对比度荧光成像。

Tags

Keywords: Three-photon MicroscopyIn VivoMouse BrainZebrafish BrainNeurological DiseasesAlzheimer’sAutismLaser SetupBrewster’s AngleAgarAnesthesiaParalysis

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