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Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
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Neuroscience
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JoVE Journal Neuroscience
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

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13,578 Views

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09:07 min

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March 17, 2014

DOI:

10.3791/51070-v

DOI:
10.3791/51070-v

•

09:07 min
March 17, 2014

•

1 Views
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1Cellular and Molelcular Neurobiology,Max Planck Institute of Experimental Medicine, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Summary

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Neuronal morphogenesis and migration are crucial events underlying proper brain development. Here, we describe methods to genetically manipulate cultured cerebellar granule neurons and the developing cerebellum for the assessment of morphology and migratory characteristics of neurons.

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Holubowska, A., Mukherjee, C., Vadhvani, M., Stegmüller, J. Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration. J. Vis. Exp. (85), e51070, doi:10.3791/51070 (2014).

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