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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

4,232 Views

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08:26 min

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January 13, 2022

DOI:

10.3791/63213-v

DOI:
10.3791/63213-v

•

08:26 min
January 13, 2022

•

19 Views
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1School of Applied and Engineering Physics,Cornell University, 2Department of Neurobiology and Behavior,Cornell University, 3College of Veterinary Medicine,Cornell University, 4Meinig School of Biomedical Engineering,Cornell University

Summary

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Three-photon microscopy enables high-contrast fluorescence imaging deep in living biological tissues, such as mouse and zebrafish brains, with high spatiotemporal resolution.

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Cite this Article

Hontani, Y., Akbari, N., Kolkman, K. E., Wu, C., Xia, F., Choe, K., Wang, G. Z., Sokolova, M., Fetcho, J. R., Xu, C. Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain. J. Vis. Exp. (179), e63213, doi:10.3791/63213 (2022).

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