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The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

DOI:
10.3791/52143-v

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08:29 min

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October 30, 2014

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,
1Institute of Neurophysiology and Cellular Biophysics and Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB),University of Göttingen

Chapters

  • 00:05Title
  • 01:20Preparation of Instruments and Pipette Fabrication
  • 02:43Preparation of Electroporation Solution
  • 03:32Electroporation of Fluorophore-coupled Dextrans
  • 05:27Mounting Animals for In Vivo Visualization of Cells and Axonal Processes
  • 06:44Results: Visualizing Olfactory Axon Projections in the Excised Olfactory Bulb
  • 08:05Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for in vivo labeling of olfactory sensory neurons by electroporation and subsequent confocal laser-scanning or multiphoton microscopy to visualize neuronal morphology and its development over time.

Tags

Olfactory SystemAxonal GrowthAxonal GuidanceNeuron MorphologyIn Vivo LabelingElectroporationFluorescent LabelingLive ImagingXenopus LaevisOlfactory EpitheliumOlfactory BulbNeural Circuit FormationSynapse Formation

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