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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

DOI:
10.3791/63213-v

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08:26 min

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January 13, 2022

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, , , , , , , , ,
1School of Applied and Engineering Physics,Cornell University, 2Department of Neurobiology and Behavior,Cornell University, 3College of Veterinary Medicine,Cornell University, 4Meinig School of Biomedical Engineering,Cornell University

Chapitres

  • 00:05Introduction
  • 01:04Three-Photon Microscopy Setup
  • 02:01Fish Preparation for Three-Photon Imaging
  • 03:52Intravital Imaging in Fish
  • 05:50Intravital Imaging in Mouse
  • 06:39Results: Three-Photon Imaging in Intact Mouse and Zebrafish Brain with High Spatiotemporal Resolution
  • 07:52Conclusion

Summary

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Traduction automatique

Three-photon microscopy enables high-contrast fluorescence imaging deep in living biological tissues, such as mouse and zebrafish brains, with high spatiotemporal resolution.

Tags

Keywords: Three-photon MicroscopyIn VivoMouse BrainZebrafish BrainNeurological DiseasesAlzheimer’sAutismLaser SetupBrewster’s AngleAgarAnesthesiaParalysis

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