Journal
/
Environment
/
Una procedura standardizzata per il monitoraggio delle fioriture algali dannose in Cile mediante analisi metabarcodifica
JoVE Journal
Environment
This content is Free Access.
JoVE Journal Environment
A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis
Please note that all translations are automatically generated. Click here for the English version.

Una procedura standardizzata per il monitoraggio delle fioriture algali dannose in Cile mediante analisi metabarcodifica

italiano

Automatically Generated

4,464 Views

•

09:47 min

•

August 26, 2021

DOI:

10.3791/62967-v

DOI:
10.3791/62967-v

•

09:47 min
August 26, 2021

•

16 Views
, , , , , , , , , , , , , , ,
1Office of Research and Academia-Government-Community Collaboration,Hiroshima University, 2Graduate School of Human and Environmental Studies,Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus,Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas,Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos,Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad,Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency,Fisheries Resources Institute

Summary

Automatically generated

Questo protocollo introduce fasi di analisi metabarcodifica, mirate ai geni rRNA 16S e rRNA 18S, per monitorare le fioriture algali dannose e il microbioma associato nei campioni di acqua di mare. È un potente strumento basato su molecole, ma richiede diverse procedure, che sono spiegate visivamente qui passo dopo passo.

Explore More Videos

Harmful Algal BloomsMetabarcoding Analysis16S RRNA18S RRNAPhytoplanktonMicroscopyPCRAgarose Gel ElectrophoresisDNA ExtractionWater SamplingMonitoring Protocol

Read Article

Cite this Article

Yarimizu, K., Fujiyoshi, S., Kawai, M., Acuña, J. J., Rilling, J., Campos, M., Vilugrón, J., Cameron, H., Vergara, K., Gajardo, G., Espinoza-González, O., Guzmán, L., Nagai, S., Riquelme, C., Jorquera, M. A., Maruyama, F. A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis. J. Vis. Exp. (174), e62967, doi:10.3791/62967 (2021).

Download .ris file

Copy

Share Video

.

Copy