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A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis
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JoVE Journal Ambiente
A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

4,450 Views

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09:47 min

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August 26, 2021

DOI:

10.3791/62967-v

DOI:
10.3791/62967-v

•

09:47 min
August 26, 2021

•

16 Views
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1Office of Research and Academia-Government-Community Collaboration,Hiroshima University, 2Graduate School of Human and Environmental Studies,Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus,Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas,Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos,Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad,Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency,Fisheries Resources Institute

Summary

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This protocol introduces steps of metabarcoding analysis, targeting 16S rRNA and 18S rRNA genes, for monitoring harmful algal blooms and their associated microbiome in seawater samples. It is a powerful molecular-based tool but requires several procedures, which are visually explained here step-by-step.

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Harmful Algal BloomsMetabarcoding Analysis16S RRNA18S RRNAPhytoplanktonMicroscopyPCRAgarose Gel ElectrophoresisDNA ExtractionWater SamplingMonitoring Protocol

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Yarimizu, K., Fujiyoshi, S., Kawai, M., Acuña, J. J., Rilling, J., Campos, M., Vilugrón, J., Cameron, H., Vergara, K., Gajardo, G., Espinoza-González, O., Guzmán, L., Nagai, S., Riquelme, C., Jorquera, M. A., Maruyama, F. A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis. J. Vis. Exp. (174), e62967, doi:10.3791/62967 (2021).

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