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Journal
/
Biochemistry
/
1
H
R
1 +
RNA 放松分散实验样品制备和设置的实用方面
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Practical Aspects of Sample Preparation and Setup of
1
H
R
1ρ
Relaxation Dispersion Experiments of RNA
Please note that all translations are automatically generated.
Click here for the English version.
1
H
R
1 +
RNA 放松分散实验样品制备和设置的实用方面
DOI:
10.3791/62470-v
•
08:17 min
•
July 09, 2021
•
Hannes Feyrer
,
Judith Schlagnitweit
,
Katja Petzold
1
Department of Medical Biochemistry and Biophysics
,
Karolinska Institutet
Chapters
00:04
Introduction
00:34
NMR Sample Preparation
03:48
1
H R
1ρ
Relaxation Dispersion
05:29
Results: Analysis of Produced Sample and Two Different Constructs Based on an RNA Hairpin
07:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个协议,测量微到毫秒的动态
13
C/15
N标签和未标记RNA与
1H
R
1+
放松分散核磁共振(NMR)光谱。此协议的重点在于高纯度样品制备和 NMR 实验的设置。
Tags
RNA Sample Preparation
NMR Setup
Proton R1Ï Relaxation Dispersion
Conformational Exchange
In Vitro Transcription
RNase H Cleavage
HPLC Purification
NMR Tube Cleaning
D2O Addition
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