Journal
/
/
Practical Aspects of Sample Preparation and Setup of 1H R Relaxation Dispersion Experiments of RNA
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Practical Aspects of Sample Preparation and Setup of 1H R Relaxation Dispersion Experiments of RNA

Practical Aspects of Sample Preparation and Setup of 1H R Relaxation Dispersion Experiments of RNA

4,255 Views

08:17 min

July 09, 2021

DOI:

08:17 min
July 09, 2021

1 Views
, ,

Summary

Automatically generated

We present a protocol to measure micro- to millisecond dynamics on 13C/15N-labeled and unlabeled RNA with 1H R relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy. The focus of this protocol lies in high-purity sample preparation and setup of NMR experiments.

Read Article