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Practical Aspects of Sample Preparation and Setup of 1H R Relaxation Dispersion Experiments of RNA
JoVE 신문
생화학
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JoVE 신문 생화학
Practical Aspects of Sample Preparation and Setup of 1H R Relaxation Dispersion Experiments of RNA

Practical Aspects of Sample Preparation and Setup of 1H R Relaxation Dispersion Experiments of RNA

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08:17 min

July 09, 2021

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08:17 min
July 09, 2021

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Summary

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We present a protocol to measure micro- to millisecond dynamics on 13C/15N-labeled and unlabeled RNA with 1H R relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy. The focus of this protocol lies in high-purity sample preparation and setup of NMR experiments.

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