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Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

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08:17 min

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July 09, 2021

DOI:

10.3791/62470-v

DOI:
10.3791/62470-v

•

08:17 min
July 09, 2021

•

2 Views
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1Department of Medical Biochemistry and Biophysics,Karolinska Institutet

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RNA Sample PreparationNMR SetupProton R1ρ Relaxation DispersionConformational ExchangeIn Vitro TranscriptionRNase H CleavageHPLC PurificationNMR Tube CleaningD2O Addition

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Feyrer, H., Schlagnitweit, J., Petzold, K. Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA. J. Vis. Exp. (173), e62470, doi:10.3791/62470 (2021).

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