Journal
/
Biochemistry
/
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

English

Automatically Generated

1,517 Views

•

07:24 min

•

September 23, 2021

DOI:

10.3791/63057-v

DOI:
10.3791/63057-v

•

07:24 min
September 23, 2021

•

8 Views
,
1Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute,The University of Texas Health Science Center at San Antonio

Summary

Automatically generated

A protocol for the application of paramagnetic relaxation enhancement NMR spectroscopy to detect weak and transient inter- and intra-molecular interactions in intrinsically disordered proteins is presented.

Explore More Videos

Paramagnetic Relaxation EnhancementIntrinsically Disordered ProteinsBiomolecular InteractionsNMR TechniquesSpin Label15N Isotopically Labeled ProteinGel FiltrationEllman’s ReagentNMR Sample PreparationPulse Calibration

Read Article

Cite this Article

Johnson, C. N., Libich, D. S. Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins. J. Vis. Exp. (175), e63057, doi:10.3791/63057 (2021).

Download .ris file

Copy

Share Video

.

Copy