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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究

DOI:
10.3791/62214-v

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08:37 min

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February 15, 2021

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, , ,
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases,IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University

Chapters

  • 00:04Introduction
  • 00:39Spleen Processing
  • 01:29T Cell Enrichment
  • 03:27iNKT Cell Enrichment
  • 05:21iNKT Cell Activation and Expansion
  • 06:35Results: Enrichment, Expansion, and Characterization of iNKT Cells
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种快速而强大的方案，以从小鼠脾脏中富集不变的自然杀伤T（iNKT）细胞，并在体外将其扩增至适合体外和体内研究的数量。

Tags

Mouse Invariant Natural Killer T CellsPurificationExpansionIn VitroIn VivoCell IsolationCell EnrichmentMACSCell StainingCell SeparationCell Culture

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