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インビトロおよびインビボ研究のためのマウス不変性ナチュラルキラーT細胞の精製と拡大
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
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インビトロおよびインビボ研究のためのマウス不変性ナチュラルキラーT細胞の精製と拡大

日本語

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3,757 Views

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08:37 min

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February 15, 2021

DOI:

10.3791/62214-v

DOI:
10.3791/62214-v

•

08:37 min
February 15, 2021

•

16 Views
, , ,
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases,IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University

Summary

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我々は、マウス脾臓から不変ナチュラルキラーT(iNKT)細胞を濃縮し、インビトロおよびインビボ研究に適した数にそれらを拡大するための迅速かつ堅牢なプロトコルを記述する。

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Cite this Article

Delfanti, G., Perini, A., Zappa, E., Fedeli, M. Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies. J. Vis. Exp. (168), e62214, doi:10.3791/62214 (2021).

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