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Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

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3,757 Views

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08:37 min

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February 15, 2021

DOI:

10.3791/62214-v

DOI:
10.3791/62214-v

•

08:37 min
February 15, 2021

•

16 Views
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1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases,IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University

Summary

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We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.

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Delfanti, G., Perini, A., Zappa, E., Fedeli, M. Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies. J. Vis. Exp. (168), e62214, doi:10.3791/62214 (2021).

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